Development of a technique for measuring the oxidation rate of a 14C-labelled substrate from 14CO2 production without the need for collection of expired air.
نویسندگان
چکیده
1. A method is proposed for the determination of the rate of oxidation of 14C-labelled substrates in man from blood sampling only. 2. The method was verified by simulating release of 14C into the bicarbonate pool of patients by using a constant-rate infusion of sodium [14C]bicarbonate, the plasma level of total 14CO2 (d.p.m. ml-1 70 kg-1) correlating well (r = 0.878, P = 0.001) with the infusion rate (microCi/h). 3. Techniques involved in employing this method are discussed, particularly in the light of errors involved.
منابع مشابه
Degradation of cellulose within the gastrointestinal tract in man.
14C-labelled cellulose was administered orally to 10 subjects without gastrointestinal disease and its absorption measured by faecal 14C excretion and 14CO2 in expired air. A mean of 57.2% of oral cellulose was excreted in the faeces and up to 14% (mean 7.5 +/- 4.4 1SD) of faecal radioactivity was water soluble. Whole gut transit time did not correlate with the quantity of 14C-cellulose excrete...
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1. Escherichia coli was grown on 14C-labelled glucose and fractions representing "whole cells", "cell contents" and "cell walls" were administered orally to rats. 2. 14C appearing as 14CO2 in the expired air and as unidentified radioactive products in urine and faeces was measured until the cumulative recovery showed little change with time. 3. All fractions were digested. The digestibility of ...
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عنوان ژورنال:
- Clinical science
دوره 60 2 شماره
صفحات -
تاریخ انتشار 1981